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. Author manuscript; available in PMC: 2010 May 15.
Published in final edited form as: Cancer Res. 2009 May 12;69(10):4107–4111. doi: 10.1158/0008-5472.CAN-09-0474

Figure 1. RIP1 regulates PTEN levels.

Figure 1

LN229 cells were infected with RIP1 adenovirus (MOI 50) or adenovirus null followed by preparation of lysates after 24h. Increased RIP1 downregulates PTEN levels and activates Akt as detected by phosphorylation of Akt at S473. B. U251MG cells (which are PTEN null) transfected with a tet inducible PTEN were exposed to tetracycline for 24h, followed by infection with RIP1 adenovirus. C. Inhibition of NF-κB using an IKBαM fails to inhibit RIP1 mediated downregulation of PTEN. LN229 cells were infected with adenovirus null or adenovirus IKBαM for 24h, followed by infection with RIP1 adenovirus for an additional 24h. D. Silencing RIP1 in LN229 cells results in an upregulation of PTEN levels. LN229 cells were transfected with RIP1 siRNA or control siRNA followed by preparation of lysates after 72h.

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