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RIP1 regulates mTOR levels in GBM cells. A. Ad-RIP1 or adenovirus null were introduced into cells for 24h followed by lysate preparation and Western blotting for mTOR, phospho-p70S6K, p70S6K, pAkt, Akt, phospho-IRS-1m IRS-1, RIP1 and Actin. B. a similar experiment was conducted in LN229 cells. C and D. Western blots showing that siRNA knockdown of RIP1 results in an increase in mTOR levels in U87MG and LN229 cells compared to control (non sequence) siRNA.
