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. 2009 Mar 6;109(2):193–205. doi: 10.1093/toxsci/kfp050

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© The Author 2009. Published by Oxford University Press on behalf of the Society of Toxicology. All rights reserved. For permissions, please email: journals.permissions@oxfordjournals.org

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FIG. 4. — The expression of genes involved in epigenetic-related processes, and those that are indicative of nuclear receptor activation, was altered by PB at 2 and 4 weeks. Microarray analysis was used to identify uniquely active genes in livers of tumor-susceptible B6C3F1 mice, as described in the “Methods.” Functional annotation of 367 uniquely active genes (Fig. 1; Supplementary Tables S3G–I) was performed. Regarding qRT-PCR analysis, a change was confirmed if the time point is underlined (data in Supplementary Tables S4–S5); gray indicates “not confirmed.” Expression of Slco1a4 was confirmed at 2, but not 4 (§) weeks. Genes noted as red or green that lack both underlining and particular symbols (+ and §) were not selected for confirmation. A plus sign (+) denotes a gene whose expression was evaluated only by qRT-PCR. White symbols represent “bridging” genes that were not altered in response to PB. Positive () or negative () regulation is illustrated, and the shapes of the entities represent the specific class of molecules to which the gene belongs: extracellular proteins () and transcription factors (). According to Ueda et al. (2002), two genes (Krt4 and Pck1) exhibited CAR-dependent downregulation in response to PB (while we detected upregulation), and three genes (¥) were under CAR-dependent “blocking,” that is, they were induced or repressed only in CAR KO mice (versus the wild-type).

Inline graphic — The expression of genes involved in epigenetic-related processes, and those that are indicative of nuclear receptor activation, was altered by PB at 2 and 4 weeks. Microarray analysis was used to identify uniquely active genes in livers of tumor-susceptible B6C3F1 mice, as described in the “Methods.” Functional annotation of 367 uniquely active genes (Fig. 1; Supplementary Tables S3G–I) was performed. Regarding qRT-PCR analysis, a change was confirmed if the time point is underlined (data in Supplementary Tables S4–S5); gray indicates “not confirmed.” Expression of Slco1a4 was confirmed at 2, but not 4 (§) weeks. Genes noted as red or green that lack both underlining and particular symbols (+ and §) were not selected for confirmation. A plus sign (+) denotes a gene whose expression was evaluated only by qRT-PCR. White symbols represent “bridging” genes that were not altered in response to PB. Positive () or negative () regulation is illustrated, and the shapes of the entities represent the specific class of molecules to which the gene belongs: extracellular proteins () and transcription factors (). According to Ueda et al. (2002), two genes (Krt4 and Pck1) exhibited CAR-dependent downregulation in response to PB (while we detected upregulation), and three genes (¥) were under CAR-dependent “blocking,” that is, they were induced or repressed only in CAR KO mice (versus the wild-type).