Table 2. Genetic diversity in the 14 peptides sequences.
| peptidea | Gene ID PlasmoDB 5.4 | SNP data extracted from PlasmoDB 5.4 | SNPs detected by sequencing of culture strainsb | ||||
| position | type of mutationc-f | frequency | position | type of mutationc-f | frequency | ||
P1 
|
PFA0170c | nt 1525 A→T nt 1518 A→G nt 1581 G→A | ns (S509G) s s | 1/15 1/15 1/15 | nt 1599 nt 1662 | Del Del | 2/13 10/13 |
| P2 TISSLSNKIVNYESKIEELEKELKEVK gabcdefgabcdefgabcdefgabcde | PFB0145c | conserved | conserved | ||||
| P5 IIDIKKHLEKLKIEIKEKKEDLENL abcdefgabcdefgabcdefgabcd | conserved | conserved | |||||
| P8 IKTMNTQISTLKNDVHLLNEQDKLNNEKGTLNSKISEL abcdefgabcdefgabcdefgabcdefgabcdefabcd NVQIMDL efgabcd | conserved | conserved | |||||
| P9 LLSKDKEIEEKNKKIKELNNDIKKL abcdefgabcdefgabcdefgabcd | conserved | conserved | |||||
| P13 LDENEDNIKKMKSKIDDMEKEIKYR abcdefgabcdefgabcdefgabcd | conserved | conserved | |||||
P14  GMNNMNGDINNINGDINNMNGDINNMNGDINNMNGDIN gabcdefgabcdefgabcdefgabcdefgabcdefgab NMN cde |
PFC0245c | nt 6662 C→T nt 6726 T→A | ns (G2221E) s | 1/10 1/8 | nt 2227 | Del (GDINNMN) | 7/13 |
| P27 KKRNVEEELHSLRKNYNIINEEIEEIT gabcdefgabcdefgabcdefgabcde | PFF0165c | conserved | conserved | ||||
| P35 ARDDIQKDINKMESELINVSNEINRLD gabcdefgabcdefgabcdefgabcde | PF14_0045 | conserved | conserved | ||||
| P38 NITNINKNIENIKNDMSNLNNMNDSNQ gabcdefgabcdefgabcdefgabcde | PF14_0089 | conserved | conserved | ||||
| P77 EKLKKYNNEISSLKKELDILNEKMGKCT fgabcdefgabcdefgabcdefgabcde | PF08_0048 | conserved | conserved | ||||
P79  NEMNKEVNKMNEEVNKMNEEVNKMNEEVNKMNKEVNKM bcdefgabcdefgabcdefgabcdefgabcdefabcde  DEEVNKMNKEVNKMNK fgabcdefgabcdefg |
PFB0315w | nt 4845 G→A | s | 1/12 | conserved | ||
P83  QNKMENDMNIIKNDMNIMENDMNIMENDMNIIKNDMNI abcdefgabcdefgabcdefgabcdefgabcdefabcd
MEKDMNIIKNDMNIIKNNMNIIKNEMNIIKNV abcdefgabcdefgabcdefgabcdefgabcd
|
PFC0345w | nt 863 T→A nt 927 G→A nt 999 A→C | ns (M288K) ns (M309I) ns (E333D) | 4/9 1/10 1/10 | nt 863 T→A nt 927 G→A nt 999 A→C nt 827 | ns (M288K) ns (M309I) ns (E333D) dup (DMNIKEN) | 5/13 1/13 1/13 4/13 |
| P90 TKKLNKELSEGNKELEKLEKNIKELEETNNTLENDIKV gabcdefgabcdefgabcdefgabcdefgabcdefgab | PFD0520c | nt 195 A→G | s | 1/9 | conserved | ||
a
Peptide sequences are aligned with the coiled coil heptad repeat (abcdefg); lines with arrows over some sequences indicate tandem repeats.
b
P. falciparum in vitro cultre strains 3D7, FC27, K1, IFA18, FV0, 7G8, HB3, ITG2F6, RO-33, W2mef, FCR3S1.2, RFCR3, MAD20
c
non synonymous (ns);
d
synonymous (s);
e
deletion (del);
f
duplication (dup).