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. Author manuscript; available in PMC: 2010 Feb 1.
Published in final edited form as: Mol Cell Neurosci. 2008 Nov 19;40(2):280–292. doi: 10.1016/j.mcn.2008.11.001

Fig. 1. Expression of VILIP-1 enhances surface expression of endogenous and co-transfected α4β2 nACh receptor in hippocampal neurons.

Fig. 1

A. VILIP-1 cDNA (pOPR-V1) or control vector (pOPR) was transfected alone (left) or co-transfected with flag-tagged α4β2 nAChR (right) into two week old primary hippocampal cultures. 16h following transfection, where no changes in cell numbers were observed, the surface expression of the endogenous α4β2 nAChR (left) or of the flag-tagged α4β2 nAChR (right) was quantified using mab299 against the α4-subunit (left) or an anti-flag antibody (right) in ELISA. B–E. Control GFP vector (B, C) or VILIP-1-GFP cDNA (D, E) were co-transfected with flag-tagged α4β2 nAChR (B–E) into 2 week old primary hippocampal cultures. After 40h in culture surface expression of the flag-tagged α4β2 nAChR (C, E) was monitored with anti-flag antibody by confocal immunofluorescence microscopy. Bar in E is 20 μm. F. Quantification of the number of cells expressing flag-tagged α4β2 nAChR at the cell surface in GFP (C) and VILIP-1-GFP (E) co-transfected hippocampal neurons. Mean values ± S.D. are from five experiments for A and three experiment for F carried out in triplicate.