Table 3.
Structure of N-linked carbohydrates in bovine TAFI
| Site | N-Glycans/Occupancy** | Observed glycopeptide mass (Da) | Theoretical glycopeptide mass (Da) | Glycan mass (Da) | Glycans*** |
| N22 | Yes/100% | 3157.2 | 3157.4 | 1622.6 | Gal2Man3GlcNAc4 |
| 3448.3 | 3448.5 | 1913.7 | Neu5Ac1Gal2Man3GlcNAc4 | ||
| 3464.3 | 3464.5 | 1929.7 | Neu5Gc1Gal2Man3GlcNAc4 | ||
| 3739.5 | 3739.6 | 2204.8 | Neu5Ac2Gal2Man3GlcNAc4 | ||
| 3755.5 | 3755.6 | 2220.8 | Neu5Gc1Neu5Ac1Gal2Man3GlcNAc4 | ||
| 4030.6 | 4030.7 | 2495.9 | Neu5Ac3Gal2Man3GlcNAc4 | ||
| 4046.7 | 4046.7 | 2511.9 | Neu5Gc1Neu5Ac2Gal2Man3GlcNAc4 | ||
| 4062.6 | 4062.7 | 2527.9 | Neu5Gc2Neu5Ac1Gal2Man3GlcNAc4 | ||
| 4321.8 | 4321.8 | 2787.0 | Neu5Ac4Gal2Man3GlcNAc4 | ||
| 4337.8 | 4337.8 | 2803.0 | Neu5Gc1Neu5Ac3Gal2Man3GlcNAc4 | ||
| 4353.8 | 4353.8 | 2819.0 | Neu5Gc2Neu5Ac2Gal2Man3GlcNAc4 | ||
| N51 | Yes/100% | 3533.6 | 3533.5 | 1622.6 | Gal2Man3GlcNAc4 |
| 3824.7 | 3824.6 | 1913.7 | Neu5Ac1Gal2Man3GlcNAc4 | ||
| 3840.7 | 3840.6 | 1929.7 | Neu5Gc1Gal2Man3GlcNAc4 | ||
| 4115.9 | 4115.7 | 2204.8 | Neu5Ac2Gal2Man3GlcNAc4 | ||
| 4131.9 | 4131.7 | 2220.8 | Neu5Gc1Neu5Ac1Gal2Man3GlcNAc4 | ||
| 4407.1 | 4406.8 | 2495.9 | Neu5Ac3Gal2Man3GlcNAc4 | ||
| 4423.1 | 4422.8 | 2511.9 | Neu5Gc1Neu5Ac2Gal2Man3GlcNAc4 | ||
| 4439.1 | 4438.8 | 2527.9 | Neu5Gc2Neu5Ac1Gal2Man3GlcNAc4 | ||
| N63 | Yes/100% | 2389.9 | 2390.0 | 1622.6 | Gal2Man3GlcNAc4 |
| 2681.0 | 2681.1 | 1913.7 | Neu5Ac1Gal2Man3GlcNAc4 | ||
| 2697.0 | 2697.1 | 1929.7 | Neu5Gc1Gal2Man3GlcNAc4 | ||
| 2972.1 | 2972.2 | 2204.8 | Neu5Ac2Gal2Man3GlcNAc4 | ||
| 2988.0 | 2988.2 | 2220.8 | Neu5Gc1Neu5Ac1Gal2Man3GlcNAc4 | ||
| 3263.3 | 3263.3 | 2495.9 | Neu5Ac3Gal2Man3GlcNAc4 | ||
| 3279.2 | 3279.3 | 2511.9 | Neu5Gc1Neu5Ac2Gal2Man3GlcNAc4 | ||
| 3295.3 | 3295.3 | 2527.9 | Neu5Gc2Neu5Ac1Gal2Man3GlcNAc4 | ||
| N86 | Yes/100% | 2851.0 | 2851.2* | 1622.6 | Gal2Man3GlcNAc4 |
| 3142.1 | 3142.3* | 1913.7 | Neu5Ac1Gal2Man3GlcNAc4 | ||
| 3158.1 | 3158.3* | 1929.7 | Neu5Gc1Gal2Man3GlcNAc4 | ||
| 3433.2 | 3433.4* | 2204.8 | Neu5Ac2Gal2Man3GlcNAc4 | ||
| 3449.2 | 3449.4* | 2220.8 | Neu5Gc1Neu5Ac1Gal2Man3GlcNAc4 | ||
| 3724.3 | 3724.5* | 2495.9 | Neu5Ac3Gal2Man3GlcNAc4 | ||
| 3740.4 | 3740.5* | 2511.9 | Neu5Gc1Neu5Ac2Gal2Man3GlcNAc4 | ||
| 3756.4 | 3756.5* | 2527.9 | Neu5Gc2Neu5Ac1Gal2Man3GlcNAc4 | ||
| N219 | unglycosylated | ||||
| N226 | unglycosylated | ||||
*Theoretical glycopeptide mass values are based on the transformation of the N-terminal Gln82 to pyroglutamic acid.
**Percent occupancy of N-glycans was determined based on MS experiments in which peptide mass fingerprints of tryptic bovine TAFI treated ± PNGase F were compared to establish the presence of (non/de)-glycosylated peptides (data not shown).
***GlcNAc, N-acetylglucosamine; Man, mannose; Gal, galactose; Neu5Ac, 5-N-acetylneuraminic acid; Neu5Gc, 5-N-glycolylneuraminic acid.