Skip to main content
. 2009 Jun;174(6):2225–2233. doi: 10.2353/ajpath.2009.080223

Figure 5.

Figure 5

mRNA expression levels of (A) eotaxin-1 and (B) MCP-3 in the dermis from wild-type mice before and at 12 and 24 hours after IgE anti-TNP Ab-mediated IC challenge. mRNA expression levels were assessed by real-time reverse transcription-PCR. Relative expression of real-time PCR products was determined by using the Ct method to compare target gene and house-keeping gene (GAPDH) mRNA expression levels. One of the control samples was chosen as a calibrator sample. The accumulation of eosinophils (C) and mast cells (D) was assessed in the skin from wild-type mice treated with polyclonal anti-MCP-3 Abs at 24 hours after IC challenge by intradermal injection of IgE anti-TNP Abs. All values represent the mean ± SEM of results obtained from 5 to 10 mice in each group.