Figure 4.

CNS-derived cells from PLPtg/PD-1^−/− mice produce higher amounts of IFN-γ⁺ at the peak of disease. IFN-γ ELISPOT assays were performed on splenocytes (A) and CNS-derived lymphocytes (B) under restimulation with MOG_35-55. A: Higher amounts of IFN-γ⁺ cells were detected in PD-1^−/− and PLPtg/PD-1^−/− mice in the early phase of EAE (day 7). B: IFN-γ⁺ cells are significantly more frequent in PLPtg/PD-1^−/− at the peak of disease (day 13). In these experiments, lymphocytes were pooled from three mice per group. *P < 0.05.