Figure 5.

Spectratyping analysis of T-cell receptors in CNS-derived lymphocytes. The T-cell receptor repertoire of lymphocytes from the CNS of wild-type, PD-1^−/−, PLPtg, and PLPtg/PD-1^−/− (n = 3) mice in the late phase of EAE (day 18) was analyzed by spectratyping. Clonal expansions (visible as single peaks in the fragment analysis) are shown as dots (different gray scales indicate individual animals). The expanded T cells are characterized by their Vβ- and Jβ-chains (Vβ, rows; Jβ, columns). The clonal expansions occurred widely distributed over different Vβ and Jβ regions, although some domains seemed to be prone for clonal expansions in different mutant mice.