Figure 7.

Infiltrating leukocytes, TH2 cytokines, chemokines, and airway hyper-responsiveness in OVA-challenged mice. Sham-treated wild-type and EphA2-deficient mice, or OVA-challenged wild-type and EphA2-deficient mice were subjected to the measurements 24 hours after last sham treatment or OVA challenge. A–C: The numbers of total cells (A), eosinophils (B), and lymphocytes (C) that infiltrated into BAL were counted. D–G: mRNA expression levels of TH2 cytokines, IL-5 (D) and IL-13 (E), and allergic airway inflammation related chemokines, eotaxin-1 (F) and eotaxin-2 (G) in airways were measured by quantitative RT-PCR. H: Assessment of airway hyper-responsiveness to inhaled methacholine in anesthetized mice. Airway responses of sham-treated wild-type (open circle) and EphA2-deficient (open square) mice or OVA-challenged wild-type (closed circle) and EphA2-deficient (closed square) mice to increasing concentrations of methacholine were assessed by a small animal ventilator. The dose-response curves for pulmonary resistance are shown. Values are presented as means ± SEM; n = 5–8 mice per group; *P < 0.05 versus OVA-challenged wild-type mice.