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. Author manuscript; available in PMC: 2009 Dec 15.
Published in final edited form as: J Pharm Biomed Anal. 2008 Sep 7;48(5):1375–1380. doi: 10.1016/j.jpba.2008.08.033

Figure 2.

Figure 2

UV spectrum of TNB, GSH-TNB and DTNB obtained in a HPLC run of a standard sample containing GSH (15 μg/ml) and DTNB (39.6 μg/ml). The detector was set to monitor the wavelengths from 200 to 600 nm. The composition of the mobile phase when GSH-TNB, TNB, and DTNB were eluated is estimated to be acetonitrile: 0.9% formic acid in water = 3:2, 4:1, and 4:1.