Table 1.

Non-protein thiols, disulfides, and GSH determined in OVCAR-3 cells. The data are presented as the means ± S.D. of three independent determinations of the same sample.

Thiols
Cell lysate supernatant	Non-protein thiols (NP-SH)a	GSH (nmol/million cells)
Before NaBH4 reduction	12.81±0.25	12.16±0.05
After NaBH4 reduction	15.77±0.41	13.52±0.04
Disulfides
Non-protein disulfidesb	Non-protein disulfides derived from mixed disulfides with GSHc
1.48±0.20	0.68±0.03

^aexpressed as GSH equivalents, nmol/million cells

^b $\text{Non-protein}\text{disulfides}=\frac{(\text{NP}-\text{SH}\text{after}{\text{NaBH}}_4\text{reduction}-\text{NP}-\text{SH}\text{before}{\text{NaBH}}_4\text{reduction})}{2}$; (expressed as GSSG equivalents, nmol/million cells)

^c $\text{Non-protein}\text{disulfides}\text{derived}\text{from}\text{mixed}\text{disulfides}\text{with}\text{GSH}=\frac{(\text{GSH}\text{after}{\text{NaBH}}_4\text{reduction}-\text{GSH}\text{before}{\text{NaBH}}_4\text{reduction})}{2}$; (expressed as GSSG equivalents, nmol/million cells)