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. Author manuscript; available in PMC: 2010 Jan 1.
Published in final edited form as: Thromb Res. 2008 Sep 3;123(5):745–752. doi: 10.1016/j.thromres.2008.07.010

Figure 3.

Figure 3

(A) Thrombin-induced tyrosine phosphorylation of EphA2 was dependent on Src kinase but not ROCK activity. Confluent HUVECs were pretreated with either DMSO, PP2 or Y27632 for 10 mins and then stimulated with 1 U/ml thrombin, as indicated by “+”. EphA2 was immunoprecipitated from clarified cell lysates using an EphA2-polyclonal antibody. Tyrosine phosphorylation was detected by western blot using the 4G10 anti-phosphotyrosine antibody. The blot was stripped and reblotted with the EphA2 antibody for loading. Representative data from 2 independent experiments were shown. (B) Mechanisms of activation of EphA2 by thrombin and by EphrinA1 were distinct. Samples were prepared as in (A), except EphrinA1 was also used as a stimulus.