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. Author manuscript; available in PMC: 2010 Jan 1.
Published in final edited form as: Thromb Res. 2008 Sep 3;123(5):745–752. doi: 10.1016/j.thromres.2008.07.010

Figure 5.

Figure 5

Overexpression of mouse EphA2 rescues thrombin-induced ICAM-1 upregulation in HUVECs with endogenous EphA2 knocked down. Either GFP or mouse EphA2 was stably overexpressed in HUVECs via retroviral infection. GFP or mouse EphA2 overexpressing cells were treated with either a control or a human EphA2 specific siRNA. ICAM-1 upregulation was then induced with 1U/ml thrombin (T) for 6 hours. Expression levels of EphA2, thrombin-induced ICAM-1 expression, and protein loading were examined by western blot analyses using antibodies specific to EphA2, ICAM-1, and α-actinin, respectively. Representative data from 4 experiments are shown.