(A) Soluble EphA2 did not block thrombin-induced ICAM-1 expression in HUVECs. Confluent HUVECs were treated with 1U/ml thrombin (T) for 6 hours in the presence or absence of 15μg/ml soluble EphA2. Expression level of thrombin-induced ICAM-1 expression and protein loading was examined by western blot analyses using antibodies specific to ICAM-1 and α-actinin, respectively. Representative results from two experiments are shown. (B) EphrinA1 did not induce ICAM-1 expression. Confluent HUVECs were either untreated, thrombin- (T, 1U/ml), or EphrinA1-treated (A1, 250ng/ml) for 6 hrs. Ephrin A1 did not induce ICAM-1 expression, whereas at the same concentration it activated EphA2 (See Figure 2). In fact, EphrinA1 up to 10 μg/ml did not induce ICAM-1 expression (not shown). Representative results from three experiments are shown.