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. Author manuscript; available in PMC: 2010 Jan 1.
Published in final edited form as: Thromb Res. 2008 Sep 3;123(5):745–752. doi: 10.1016/j.thromres.2008.07.010

Figure 8.

Figure 8

Endothelial EphA2 was crucial in mediating leukocyte attachment to thrombin-stimulated HUVECs. Either GFP or mouse EphA2 was stably overexpressed in HUVECs via retroviral infection. GFP or mouse EphA2 overexpressing cells were treated with either a control or a human EphA2 specific siRNA. Confluent HUVECs were stimulated with 5U/ml thrombin for 6 hours. Fluorescently labeled U937 cells were then added. After one hour of incubation at room temperature, unattached cells were gently aspirated away. Cells were then fixed in 4% PFA. (A) U937 cells were detected by fluorescence microscopy. Experiments were done in triplicate. Each condition was done three times. Representative results are shown. (B) Number of attached U937 cells were counted in 4 randomly chosen fields of each experiments. Three experiments per condition were done. Data are mean ± s.d. of three experiments; * p<0.005; # p<0.02.