Fig. 3.

Cytokine production of TCR-transduced PBLs following co-culture with cells expressing the cognate antigen. a PBL transduced with supernatant from packaging cell clones APB 38, GSGP2A 3, GSGT2A 36, and T2A 24 were co-cultured with T2 cells pulsed with NY-ESO-1 peptide (p157-165v) or influenza-specific peptide. Antigen-specific IFN-γ secretion by retroviral vector-transduced PBLs was determined by ELISA. Data are the mean values (picograms per milliliter) of duplicate samples, with standard deviation (error bars). b PBL were transduced with supernatant from retroviral constructs for GFP (control virus) or from APB, GSGP2A, GSGT2A, and T2A and were then co-cultured with HLA-A2+, NYESO-1+ melanoma lines (624.38mel, 1300mel, A375mel) or HLA-A2+, NY-ESO-1- melanoma line (SK23). Transduced PBL were also incubated with T2 cells alone, or T2 cells pulsed with NY-ESO-1 peptide (p157-165v) or gp100 peptide. Antigen-specific IFN-γ secretion by retroviral vector-transduced PBLs was determined by ELISA. Data are the mean values (picograms per milliliter) of samples, with standard deviation (error bars)