Table 1. Primer sequences used for qRT-PCR.

Gene GenBank No.	Primer Sequence (5′-3′)	Amplicon (bp)	Tm (°C)
Claudin-1 NM_021101
Forward	GAA GTG CTT GGA AGA CGA TGA G	190	56
Reverse	GCC CAG CCA GTG AAG AAG G
Claudin-5 NM_003277
Forward	CTC TGC TGG TTC GCC AAC AT	87	57
Reverse	CAG CTC GTA CTT CTG CGA CA
E-selectin NM_000450
Forward	TGA GAC AGA GGC AGC AGT G	199	57
Reverse	CCG TGG AGG TGT TGT AAG AC
GAPDH BC025925
Forward	AGT TAG CCG CAT CTT CTT TTG C	96	57
Reverse	CAA TAC GAC CAA ATC CGT TGA CT
ICAM-3 NM_002162
Forward	AGC GGC AGT TAC CAT GTT AGG G	146	58
Reverse	GGC TTT ATT GGT GCG GAA TCT GAG
Occludin DQ892423
Forward	TCC AGA GTC TTC CTA TAA ATC CAC	106	57
Reverse	ACC ACC GCT GCT GTA ACG
PECAM NM_000442
Forward	GCA GTC TTC ACT CTC AGG ATG	143	57
Reverse	GGC AGG CTC TTC ATG TCA AC
VCAM-1 BC068490
Forward	TGC TGC TCA GAT TGG AGA CTC	113	55
Reverse	TCC TCA CCT TCC CGC TCA G
ZO1 NM_003257
Forward	GAA TGA TGG TTG GTA TGG TGC G	191	60
Reverse	TCA GAA GTG TGT CTA CTG TCC G
WNV (ENV) NC_009942
Forward	CAG ACC ACG CTA CGG CG	112	60
Reverse	CTA GGG CCG CGT GGG
Probe	TCT GCG GAG AGT GCA GTC TGC GAT