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. 2009 May 19;106(22):9099–9104. doi: 10.1073/pnas.0900591106

Fig. 2.

Fig. 2.

Impaired induction of mucin, cytokines, and chemokines in OVA-challenged airways of Trpa1-deficient mice. (A) Relative quantities (RQ) of mucin5ac gene transcript, determined by Taqman qPCR of whole mouse lung cDNA. Mucin5ac induction is diminished in Trpa1−/− OVA mice. GAPDH transcript levels were used for normalization as endogenous control. Animal groups: Trpa1+/+ veh: n = 4, Trpa1−/− veh: n = 4, Trpa1+/+ OVA: n = 6, Trpa1−/− OVA: n = 7. ∗, P < 0.05. (B) Relative quantities (RQ) of interleukin 5 (IL-5) gene transcript, as determined by Taqman real-time quantitative PCR of whole mouse lung cDNA. OVA-challenged Trpa1−/− mice show no significant changes in IL-5 transcription compared with vehicle-treated mice. GAPDH transcript levels were used for normalization as endogenous control. Animal groups: Trpa1+/+ veh: n = 4, Trpa1−/− veh: n = 4, Trpa1+/+ OVA: n = 6, Trpa1−/− OVA: n = 6. ∗∗∗, P < 0.001. (C) Comparison of cytokine and chemokine levels in BALF of OVA-challenged Trpa1+/+ (white) and Trpa1−/− (black) mice, as measured by Luminex peptide analysis. Groups: Trpa1+/+ n = 8–10, Trpa1−/− n = 8–10 for each analyte. ∗∗, P < 0.01; ∗∗∗, P < 0.001