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Nondenaturing polyacrylamide gels indicating the degree of oligomerization of DtxR(130–226) (lane A) and DtxR(144–226) (lane B). The reduced electrophoretic mobility of DtxR(144-226) monomer compared to DtxR(133-226) monomer reflects the additional residues at the N terminus of DtxR(144-226) arising from the expression construct (see Materials and Methods).
