Fig. 2.
Small increases in [Ca2+]I are required for hypoxia to induce ischemic tolerance in hippocampal slice cultures. A. BAPTA-AM buffers increases in [Ca2+]i during hypoxic preconditioning. * significant increase in [Ca2+]i (P<0.05) compared to baseline; numbers indicate number of observations. B. BAPTA-AM present during preconditioning eliminates induction of tolerance to oxygen and glucose deprivation (OGD). * indicates significant reduction in cell death following HPC (P<0.05). C. Representative propidium iodide fluorescence images from slices at baseline, after OGD (no preconditioning), in preconditioned slices 48 hr after OGD, and in a slice treated with BAPTA-AM before preconditioning.
