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. 2009 May 26;7(5):e1000116. doi: 10.1371/journal.pbio.1000116

Figure 5. FAK competes against Nudel for paxillin.

Figure 5

(A) Schematic diagrams of FAK and mutants. Their paxillin-binding abilities are summarized from literature [51],[52]. Their abilities to compete with Nudel for paxillin are based on results in (B). (B) Overexpression of FAK or certain mutants disrupts the Nudel-paxillin interaction. Co-IP was performed with lysates of HEK293T cells overexpressing FLAG-Nudel, GFP-Paxillin (asterisks), and a GFP fusion protein as indicated above (arrowheads). (C) Nudel associates with the LIM domain of paxillin, and the interaction is not affected by FAK. Co-IP was performed with lysates of HEK293T cells overexpressing FLAG-Nudel, GFP-tagged FAKΔFERM or FAKΔFAT (arrowheads), and GFP-tagged paxillin or a mutant indicated above (asterisks). (D) Regulation of the Nudel-paxillin interaction by endogenous FAK. Low levels of FLAG-GFP-Nudel (at 3–6-fold of the endogenous Nudel level) or FLAG-GFP were expressed in HEK293T cells transfected with either control or FAK RNAi plasmids (lanes 1–6). After co-IP, proteins associated with anti-FLAG resin were visualized by immunoblotting.