Table 2.
Differential expression of myostatin targets genes confirmed by qPCR and by western-blot experiments in MSTN-null mice vs their control littermates.
| Proteomics | Transcriptomics | qPCR | Western-Blot | |
| H-FABP | -2.1 | -9.1 | ||
| MYBPH | 3.1 | 1.4 | ||
| MyHCII | 4.9 | |||
| Syntrophin A | 1.5 | 2.8 | ||
| p-Akt | 2.2 | |||
| GSK-3β | -1.9 | -2.4 | ||
| GSK-3β(ser9)/GSK-3β | 1.7 t | |||
| PI3KR3 | 1.1 | 1.8 | ||
| DJ-1 | 1.5 | 1.7 | 2.2 | |
| PINK1 | 1.9 | 3.4 | ||
| PTEN | -1.6 t | -1.6 | ||
| Mortalin | -1.5 | -1.4 | -2.1 | |
| TCTP | 2.7 | 1.6 | ||
| 14-3-3E | 1.8 | 1.4 | 1.7 | |
| mfgf6 | 1.9 | |||
Results are expressed as Fold Changes and all the differences have been declared significant (P < 0.05) except for some tendencies (t: P < 0.10).
H-FABP: Heart-Fatty acid binding protein H; MyBP-H: Myosin binding protein H; MyHCII: fast myosin heavy chain isoform; pAkt: phosphorylated Akt; GSK-3β: Glycogen synthase kinase 3β; GSK-3β (ser9): Glycogen synthase kinase 3β (phosphorylated at serine 9); PI3KR3: Phosphatidylinositol 3-kinase regulatory subunit; DJ-1: cancer- and Parkinson's disease-associated protein (park7); PINK1: PTEN-induced putative kinase 1; PTEN: tumour-suppressor phosphatase with tensin homology. TCTP: Translationally controlled tumor protein; 14-3-3E: 14-3-3ε protein; mfgf6: murine Fibroblast growth factor 6.