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. Author manuscript; available in PMC: 2009 May 19.
Published in final edited form as: Endocrine. 2008 Apr 8;33(1):32–39. doi: 10.1007/s12020-008-9055-0

Fig. 8.

Fig. 8

Effect of IGF-I immuno-neutralization on high glucose-induced fibronectin and IGFBP-2 production. MES-13 cells were cultured under basal (5.5 mmol/l) or high (25 mmol/l) glucose conditions without (“C” = controls) or with IGF-I antiserum added at 1:10,000 or 1:5,000 titers. Fibronectin levels were measured using ELISA, as described in Methods; bars represent mean ± SE values (n = 6/mean), with different a,b,c superscripts indicating significantly different values (P < 0.05). IGFBP-2, measured by Western immunoblot procedure, is shown in the lower panels. Immuno-neutralization of IGF-I inhibits the high glucose-induced increases in fibronectin and IGFBP-2