Fig. 9.
Effect of IGF-I immuno-neutralization on Ang II-induced fibronectin and IGFBP-2 production. MES-13 cells were cultured under basal glucose conditions (5.5 mmol/l; “C” indicates non-hormone-treated control) and treated with 10−7 or 10−6 M Ang II in the absence [“(−)” lanes] or presence [“(+)” lanes] of IGF-I antiserum at a titer of 1:5,000. Fibronectin levels (upper panel) were measured using ELISA, as described in Methods; bars represent mean ± SD values (n = 6/mean), with a,b superscripts indicating significantly different values (P < 0.05). IGFBP-2, measured by Western immunoblot procedure, is shown in the lower panels. Immuno-neutralization of IGF-I inhibits Ang II-induced increases in fibronectin and decreases IGFBP-2 to non-detectable levels