Figure 2. OGR1 expression in mouse tissues and cells.

(A) OGR1 expression in various tissues from FL and KO mice detected by RT-PCR. The PCR program was 94°C, 2 min; 25 cycles for actin and 35 cycles for OGR1 (94°C, 30 s; 55°C, 1 min; 72°C, 1 min); 72°C, 10 min, except both actin and OGR1 in macrophages were amplified by 30 cycles. BAT, brown adipose tissue; BM, bone marrow; PBL, peripheral blood leucocytes. The arrows indicate DNA ladders with 500 bp. (B) OGR1 distribution in lung tissue from FL and C57/BL6 mice. Anti-FLAG M2 antibody (1∶200) was used. Representative positively stained cells are indicated by arrows. Scale bar = 100 µm.