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. 2009 May 28;4(5):e5698. doi: 10.1371/journal.pone.0005698

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Riazi et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Quantitative RT-PCR analysis of Nkx2-5, Gata4, and β-catenin in the hearts of 11.5 dpc wild type (WT) and Nkx2-5^+/- (HET) embryos revealed augmentation of both Gata4 and β-catenin and reduction in Nkx2-5 RNA levels. The values (mean±SEM) for each gene were normalized against GAPDH. N = 9 for WT and N = 11 for HET hearts.