Figure 4. Strategy with prolonged subculture applied to co-culture of MTAP-negative MCF-7 and MTAP-positive HF cells.

MCF-7 and HF cells were co-cultured with drugs added as follows: (A) Start of co-culture. (B) Four days with DAP (100 µM) and MTA (25 µM). (C) Co-culture in panel B split 1:5 into drug-free medium and fixed two days later. (D) Same as in panel C except culture fixed after five days. Representative fields are shown.