Figure 5. TH mRNA 3′UTR conferred cAMP-mediated inducibility on the luciferase gene in MN9D cells.

(A) Diagrams of the TH-luciferase constructs used in these experiments are shown in the figure. (B) MN9D cells were transfected with one of the TH-luciferase constructs along with the Renilla luciferase expression vector used for normalization purposes. Twenty-four hr after transfection, the cells were treated with or without 0.5 mM 8-CPT-cAMP for another 24 hr. The cells were then harvested and firefly and Renilla luciferase activities were measured in cell extracts. The results represent the means ± SE from 11-12 dishes, except for the modified GL3 data which were from 4 dishes. a: p < .01 compared to controls. (C) PC12 or cath.a cells were transfected with the TH(3′U)-luciferase and the Renilla expression vector constructs. Twenty-four hr after transfection, the cells were treated for another 24 hr with or without either 1 mM 8-CPT-cAMP (cath.a cells) or 10 uM forskolin (PC12 cells). The results represent the means ± SE from 4-5 dishes.

a: p < .01 compared to controls