Figure 6. TH-luciferase mRNA levels were not increased by treatment of transiently-transfected MN9D cells with 8-CPT-cAMP.

MN9D cells were transfected with TH(3′U)-luciferase and the Renilla luciferase expression vector. Twenty-four hr after transfection, the cells were treated for another 24 hr with or without 0.5 mM 8-CPT-cAMP. The cells were then harvested and total cellular RNA was isolated. The RNA samples were treated with 2 units of TURBO-DNase (unless otherwise stated) prior to RT-PCR. (A) Autoradiograms showing both firefly and Renilla luciferase mRNA levels detected by semiquantitive RT-PCR with or without RT are shown in the figure. (B) Autoradiograms are shown depicting semiquantitative RT-PCR assays run under different conditions as noted in the figure measuring firefly and Renilla luciferase mRNAs, along with TH mRNA and 28S rRNA in the same samples. (C) Results are shown of 4 experiments in which both luciferase activity and luciferase mRNA levels expressed in MN9D cells transiently-transfected with the TH(3′U)-luciferase construct were measured in the same samples. The results represent the means ± SE from 17-18 dishes.

a: p < .01 compared to controls.