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. Author manuscript; available in PMC: 2009 May 20.
Published in final edited form as: Protein Expr Purif. 2006 Dec 24;53(1):24–30. doi: 10.1016/j.pep.2006.11.022

Figure 1.

Figure 1

M. tuberculosis membrane proteins were expressed as fusions to the C-terminus of non-secreted MBP. The fusion construct has eight histidines and a thrombin recognition and cleavage site positioned between MBP and the fused polypeptide. The thrombin recognition site is composed of LVPRGS amino acids. Two amino acid residues (glycine and serine) remain with the N-terminus of the expressed protein after thrombin digest. Molecular weight of MBP with the C-terminal His-tag generated after thrombin digest is 42.5 kDa.