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. Author manuscript; available in PMC: 2009 May 20.
Published in final edited form as: Protein Expr Purif. 2006 Dec 24;53(1):24–30. doi: 10.1016/j.pep.2006.11.022

Figure 4.

Figure 4

Polyacrylamide gel results of a thrombin digest of Rv1824 MBP fusion protein using gels as described in Figure 2. Thrombin digest results in two protein bands on the gel: 12.7 kDa for Rv1824 and 42.5 kDa for MBP with the addition of the His-tag. The lanes are as follows: NC – not cleaved; MW – molecular weight marker; SDS – 0.01% SDS added to reaction mixture; Urea – 0.8M urea added to reaction mixture; U – units of thrombin per mg fusion protein added to reaction mixture. In all cases, reaction mixture was incubated at room temperature for 14 hours.