Table 1.

Relative expression levels shown as observed on Coomassie-stained denaturing polyacrylamide gels: “o” denotes no observed expression, and “+” though “++++” represents increasing expression level, where “+” corresponds to an expression yield between 10 mg and 30 mg of fusion protein per L of the bacterial culture, “++” corresponds to yields between 40 and 60 mg, “+++” corresponds to fusion protein yields between 70 and 90 mg, and “++++” corresponds to yields of fusion protein exceeding 100 mg. The amount of protein was determined by absorbance at 280 nm after eluting the sample from an amylose or metal affinity column. Abbreviations are as follows: #TM – the number of putative transmembrane helices; MW – molecular weight in kDa; n/a – not applicable; n/t – not tested.

Membrane protein from M. tuberculosis	Fusion protein MW, kDa	Membrane protein MW, kDa	# TM	LB media growth		Autoinduction media growth		Thrombin Digest, %
				sol	insol	sol	insol	sol	insol
Rv3656c	49.6	7.1	1	o	o	o	o	n/a	n/a
Rv0476	51.7	9.2	2	+++	+++	+++	+++	10	n/t
Rv0514	53.0	10.5	2	o	+	+	+++	90	n/a
Rv0879c	52.1	9.6	2	+	+	+	++	100	n/a
Rv1305	50.7	8.2	2	+	++	o	+++	100	n/a
Rv1440	50.7	8.2	2	+++	+++	++++	++++	0	n/t
Rv1567c	53.0	10.5	2	+	++	+	++++	100	n/a
Rv2076c	51.6	9.1	2	o	o	o	o	n/a	n/a
Rv2128	50.0	7.5	2	+	+++	o	++++	100	n/a
Rv2144c	54.5	12.0	2	++	+	++	+	0	n/t
Rv2876	54.3	11.8	2	+	++	++	++	100	n/a
Rv3346c	51.5	9.0	2	o	++++	o	++++	n/a	30
Rv0460	50.7	8.2	3	o	+	+	++	100	n/a
Rv0463	52.7	10.2	3	+	+++	+	++++	100	n/a
Rv0882	52.3	9.8	3	+	+	+	+	100	n/a
Rv0961	54.9	12.4	3	o	o	o	o	n/a	n/a
Rv3155	53.4	10.9	3	o	++	o	+++	n/a	50
Rv3355c	52.8	10.3	3	o	+	o	++	n/a	50
Rv1824	55.2	12.7	4	+	++	+++	+++	100	n/a
Rv2600	56.1	13.6	4	o	o	o	o	n/a	n/a
Rv3065	53.6	11.1	4	o	o	o	o	n/a	n/a
Rv3070	55.7	13.2	4	o	o	o	o	n/a	n/a