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Mutant SIVmac239 Nef TW9 infects and replicates in rhesus macaque CD4+ T cells at levels comparable with WT SIVmac239 Nef IW9 virus in vitro. CD4+ T cells from a rhesus macaque infected with WT SIVmac239 (Nef IW9) or the mutant Nef TW9 virus were cultured in 24-well tissue culture plates at a cell density of 1 × 106 cells/mL. The frequency of SIV Gag p27 expressing CD4+ T cells was determined at 24, 72, 120 and 168 hours post infection by flow cytometry.
