Table 1.
Intracellular cytokine responses to wild type and variant peptides
| SIV CTL epitope | Peptide sequencec | % IFN-γ producing cellsd |
|---|---|---|
| Gag CM9a | CTPYDINQM (wild type) | 85.2 |
| CAPYDINQM (T2A) | 0.1 | |
| CSPYDINQM (T2S) | 4.1 | |
| Tat SL8a | STPESANL (wild type) | 75.4 |
| PTPESANL (S1P) | 1.4 | |
| Nef IW9b | IRYPKTFGW (wild type) | 37.7 |
| TRYPKTFGW (I1T) | 39.2 |
a
Mamu A*01-restricted epitope.
b
Mamu B*17-restricted epitope.
c
Wild type peptide sequence and observed mutations in CTL epitopes in virus isolated from a chronically infected rhesus macaque.
d
Frequency of cells positive for IFN-γ in clonal population of CTL generated against WT peptide after stimulation with APC pulsed with 5 μg/mL of WT or variant peptide; analyses by ICS and flow cytometry.