Table 2.
Quantification of virus
Treatmenta | Proviral DNAb | Viral RNAc |
---|---|---|
Uninfected cells | d≤6.7×102 | d≤1.5×103 |
Nef IW9 infected cells | 1.5×108 | 5.4×109 |
Nef TW9 infected cells | 2.2×108 | 5.5×109 |
Rhesus macaque CD4+ T cell clone, uninfected, infected with wild type SIVmac239 Nef IW9 or mutant Nef TW9.
DNA copies/100,000 cells determined by QPCR.
RNA copies Eq/mL cell culture supernatants determined by RT PCR. Analyses performed on day 8 post infection.
Assay sensitivity threshold.