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. 2009 Apr 24;7:37. doi: 10.1186/1477-7827-7-37

Figure 1.

Figure 1

The level of Cyp19a1 mRNA in 10-week-old mouse tissues measured by real-time RT-PCR. Cyp19a1 and GAPDH cDNAs from mouse ovary were cloned into pCR®II-TOPO plasmid vector. Different concentrations (10-1 to 10-12 μg DNA/μl) of plasmid DNAs containing Cyp19a1 or GAPDH sequence were amplified with primers for Cyp19a1 or GAPDH as the external standard curves. The ratio of copy numbers of Cyp19a1 (× 107) to copy numbers of GAPDH was calculated as Cyp19a1 mRNA levels. Pituitary was analyzed from pools of 5 animals. Data are expressed as mean ± SE with n = 5 mice in each group.