Table 2. Transcripts differentially expressed in sleep restriction relative to migration and spontaneous wakefulness.
White-crowned sparrow telencephalon genes whose mRNA levels are changed in 78 hours of sleep restriction (SR) relative to migration (M) and 6 hours of spontaneous wakefulness (W). (a) transcripts increased (>1.2 fold change, Welch t-test, p<0.05) (b) transcripts decreased (<1.2 fold change, Welch t-test, p<0.05) “% change (array)”, percentage change in SR relative to M and W as indicated by array analysis. “% change (PCR)” indicates transcripts confirmed using qPCR from pooled RNA of 4 additional birds not previously used for microarray analysis.
| A. Transcripts with higher expression in sleep restriction relative to migration and spontaneous wakefulness | ||||
|---|---|---|---|---|
| Genbank | Gene description | Gene symbol | % Change (array) | % Change (PCR) |
| IMMUNE FUNCTION | ||||
| DQ214165 | immunoglobulin lambda light chain | IGLL | 1.49;1.25 | 54.1;40.6 |
| DQ214165 | immunoglobulin lambda light chain | IGLL | 1.36;1.61 | |
| MISCELLANEOUS | ||||
| NM_001031050 | NIMA (never in mitosis gene a)-related kinase 2 | 1.21;1.34 | ||
| NM_204232 | HIR histone cell cycle regulation defective homolog A | HIRA | 1.78;1.76 | 67.9;23.1 |
| NM_001012824 | mannosyl (alpha-1,3-)-glycoprotein beta-1,4-N-acetylglucosaminyltransferase, isozyme A | MGAT4A | 1.33;1.24 | |
| XM_420329 | SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 1 | SMARCA1 | 1.46;1.57 | |
| XM_001231767 | ATPase, Ca++ transporting, plasma membrane 2 | ATP2B2 | 1.40;1.31 | 9.3;0.12 |
| B. Transcripts with lower expression in sleep restriction relative to migration and spontaneous wakefulness | ||||
| GenBank | Gene description | Gene symbol | % Change (array) | % Change (PCR) |
| ENZYMES | ||||
| DQ214020 | Glutathione peroxidase (Taeniopygia guttata) | 1.47;1.54 | 53.9;12.02 | |
| NM_204259 | prostaglandin D2 synthase 21kDa | PTGDS | 1.30;1.21 | 29.06;31.5 |
| TRANSCRIPTION FACTORS | ||||
| XM_417039 | general transcription factor IIF | GTF2F2 | 1.38;1.40 | |
| MISCELLANEOUS | ||||
| NM_001004376 | hemoglobin, alpha 1 | HBA1 | 1.45;1.34 | |
| NM_001004375 | hemoglobin, alpha 2 | HBA2 | 1.38;1.29 | 22.8;40.4 |
| NM_001004375 | hemoglobin, alpha 2 | HBA2 | 1.22;1.32 | |
| NM_001004376 | hemoglobin, alpha 1 | HBA1 | 1.52;1.67 | |
| NM_001004376 | hemoglobin, alpha 1 | HBA1 | 1.32;1.25 | |
| XM_419213 | proenkephalin | PENK | 1.21;1.25 | |