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. 1999 May 25;96(11):6137–6142. doi: 10.1073/pnas.96.11.6137

Figure 4.

Figure 4

Aggregation of α-lactalbumin after the addition of 20 mM DTT at 37°C and pH 6.7. Curve 1, 0.25 mg of α-lactalbumin; curve 2, 0.25 mg of α-lactalbumin plus 0.25 mg of wild-type αB-crystallin; curve 3, 0.25 mg of α-lactalbumin plus 0.25 mg of R120G αB-crystallin. The Inset is an SDS/polyacrylamide gel that illustrates the particulate and soluble fractions obtained after a 60-min incubation of R120G αB-crystallin with α-lactalbumin in the presence of DTT, as shown in curve 3. Lane A, αB-crystallin; lane B, α-lactalbumin; lane C, soluble fraction of α-lactalbumin plus R120G αB-crystallin; lane D, particulate fraction of α-lactalbumin plus R120G αB-crystallin.