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. Author manuscript; available in PMC: 2009 May 20.
Published in final edited form as: Dev Biol. 2008 Feb 13;317(2):649–659. doi: 10.1016/j.ydbio.2008.01.036

Fig. 3. Major CNS enhancers are located upstream of CiPax6 coding sequence.

Fig. 3

Photomicrographs of β-galactosidase (β-gal) histochemical assays of lacZ expression driven by various reporter transgenes. Mid-tail (upper panels, A, C, E, G, I, K) and late tail (lower panels, B, D, F, H, J, L) embryos in lateral view with anterior to the left, except as noted. Transgene name and diagram are located below each embryo pair. (A, B) Expression from upstream, intron 1, and intron 4 fragments together. β-gal staining is seen in the entire CNS (arrowheads). Expression is also seen in 2 muscle cells on each side of the trunk, derived from the A8.16 lineage (arrows, see text). These cells also stain with the CiP6-2.5U, -2.0U, and -1.8U constructs. (C, D) 2.5 kb of upstream sequence alone drives expression in the entire CNS, as well as ectopic expression in epidermis (arrows) (D inset) dorsal detailed view of trunk of late tail CiP6-2.5U embryo. (E, F) Specific expression in the sensory vesicle (sv) is driven by a construct with 1986 bp. of upstream sequence. At mid tailbud stage sporadic expression is also seen in the caudal nerve cord (arrowheads) (F inset) Right lateral view of late tail embryo with CiP6-2.0U transgene expression detected with an anti-lacZ antibody. Expression can be seen in the sensory vesicle surrounding the pigmented otolith and ocellus. A cell in the caudal visceral ganglion (arrowhead) also reacts with the lacZ antibody (white arrowhead). (G, H) Deletion of 211 bp. containing the UA CNE results in loss of most CNS expression except for slight expression in the caudal nerve cord at mid tailbud stage (arrowheads). Ectopic expression in mesenchyme appears in this truncated construct (arrows). (I, J) Addition of the distal sequence containing the UB CNE to the -1.8U construct results in gain of some sensory vesicle expression (white arrowheads) in addition to the low level expression in the nerve cord (arrowhead). This construct exhibits some ectopic expression in tail muscle cells at late stages (arrows). (K, L) A construct with only 0.6 kb. of upstream sequence shows only ectopic β-gal expression in trunk mesenchyme at late stages (arrows).