Table 1. Region-specific mRNA expression due to various intron plus upstream non-coding sequence reporter transgene combinations, as assessed by WMISH.
Stage | Anatomical region | % of embryos with reporter expression in region listed | |||
---|---|---|---|---|---|
CiP6-2.5U | CiP6-2.5UI1 | CiP6-2.5UI4 | CiP6-2.5UI1I4 | ||
Mid tailbud stage | Sensory vesicle | 100 | 100 | 100 | 100 |
Visceral ganglion (ectopic) | 58 | 87 | 20 | 16 | |
Nerve cord | 67 | 100 | 7 | 42 | |
n=12 | n=23 | n=30 | n=19 | ||
Late tailbud stage | Sensory vesicle | 100 | 100 | 100 | 90 |
Visceral ganglion (ectopic) | 30 | 80 | 7 | 10 | |
Nerve cord | 20 | 80 | 13 | 40 | |
n=10 | n=10 | n=15 | n=10 |
Refer to Fig. 2B for diagrams describing the reporter transgene constructs.