Fig. 3.

The p120RasGAP SH2-SH3-SH2 (2-3-2) region bridges FAK and p190A. (A) Schematic representation of the mammalian GST expression vectors of GST–2-3-2 and GST–3-2 of p120RasGAP. (B) Coexpression of GST–2-3-2 and FAK facilitate the formation of a complex with p190A. FAK^–/– MEFs were transiently transfected with the indicated GST constructs (left) or GST constructs plus GFP-FAK (right), plated (30 minutes) onto FN-coated dishes and lysates were analyzed by glutathione-agarose bead pull down. Association of endogenous Pyk2, p190A or GFP-FAK was determined by immunoblotting.