Fig. 3.

Purified His₆-VASP interacts with amino acids 331-355 from the CXCR2 C-terminus. (A) 96-well plates were coated with 3 μg/ml GST, GST-CXCR2 311-330, or GST-CXCR2 331-355 and incubated with various concentrations of His₆-VASP (0.06-60 ng/ml). Bound His₆-VASP was detected using nickel-activated HRP (Abs 405 nm). Triplicate experiments show His₆-VASP binding (mean ± s.e.m.) to GST-CXCR2 331-355 was statistically higher than to GST alone or GST-CXCR2 311-330 (*P≤0.05, Mann Whitney U-test). Data shown are representative of at least three separate experiments. (B) Representative Coomassie-blue-stained gel of 5 μg purified GST, GST-CXCR2 311-330 and GST-CXCR2 331-355. (C) Representative Coomassie-blue-stained gel of 5 μg purified His₆-VASP.