Fig. 4.

VASP is phosphorylated on Ser157, Ser239 and Thr278 specifically in response to CXCL8 stimulation. HL-60-CXCR2 cells were stimulated with vehicle (Untreated) or 100 ng/ml CXCL8 for 1 or 5 minutes. Lysates were subjected to SDS-PAGE and western blot analysis (IB) using antibodies specific for Ser157-P (A), Ser239-P (B) or Thr278-P VASP (C). HL-60-CXCR2 cells were stimulated with vehicle (Untreated) or 100 ng/ml CCL3 for 1 minute. Lysates were subjected to SDS-PAGE and western blot analysis (IB) for Ser157-P (D), Ser239-P (E) or Thr278-P VASP (F). Data shown are representative of three separate experiments.