Fig. 4.

Increased mortality and weight loss in rJ.M_Y135Q-infected mice after Treg depletion. (A) Mice were treated with 0.5 mg anti-CD25 antibody (mAb PC61) or rat IgG 3 days prior to infection with rJ.M_Y135Q. Percentage of Foxp3⁺CD25⁺ of total CD4 T cells in the brain at day 5 p.i. (8 days post treatment) is shown. (B, D) B6 mice were infected with rJ.M_Y135Q three days after treatment with anti-CD25 antibody (n = 10) or rat IgG (n =15) and monitored for survival (B) and weight loss (D). Results from three independent experiments are shown. Mortality (p < 0.05) and weight loss (p < 0.05 at days 8–13) were significantly increased in anti-CD25 mAb-treated mice. (C) Viral titers in the brain at day 5 p.i after treatment with mAb PC61 or rat IgG (n = 5 for each antibody).