Antibody cross-linking of C-D2R does not immobilize D2R-V. A, recovery of
C-D2R fluorescence after photobleaching (at time = 10 s) in cells expressing
C-D2R and D2R-V is nearly abolished by anti-GFP antibody cross-linking; lines
represent the mean of all experiments, gray lines represent the mean ±
S.E.M. B, recovery of D2R-V fluorescence in the same cells as A. C to E,
recovery of normalized fluorescence (measured at time = 180 s) for cells
expressing C-D2R and D2R-V (C; control, n = 59; anti-GFP, n
= 61), C-D2R and β2AR-V (D; control, n = 15; anti-GFP,
n = 16), and C-TM and D2R-V (E; control, n = 9; anti-GFP,
n = 9); *, P < 0.002; **,
P < 0.001; bars represent mean ± S.E.M.