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. 2009 Mar 9;75(6):1296–1299. doi: 10.1124/mol.108.053876

Fig. 3.

Fig. 3.

Oxidative cross-linking stabilizes C-D2R-D2RV hetero-oligomers via Cys168. A, immunoblot probed with anti-GFP primary antibody of lysates from cells transfected with equal amounts of C-D2R or D2R-V (both Cys168 or Ser168) plasmid DNA, with or without CuP treatment; the predicted molecular mass of both protomers is ∼80 kDa. B and C, recovery of D2R-V fluorescence after antibody cross-linking alone (anti-GFP) or antibody and oxidative cross-linking (+CuP); lines represent the mean ± S.E.M. of all cells expressing C-D2R Cys168 and D2R-V Cys168 (B) or C-D2R Cys168 and D2R-V Ser168 (C). D to F, recovery of normalized fluorescence for cells expressing (measured at time = 180 s) for C-D2R Cys168 and D2R-V Cys168 (D; control, n = 46; CuP, n = 48), C-D2R Cys168 and D2R-V Ser168 (E; control, n = 25; CuP, n = 31), and C-D2R Ser168 and D2R-V Cys168 (F; control, n = 11; CuP, n = 17); **, P < 0.001; n.s., P > 0.1; bars represent mean ± S.E.M.