Fig. 10.

Quantification of CYP4F2 in genotyped HLM. Samples were subjected to Western blotting with anti-CYP4F2 IgG as described under Materials and Methods. Lanes 1 and 5 contain a 55-kDa molecular mass standard. Lanes 2, 3 and 4 contain 1.0, 0.5, and 0.1 pmol of recombinant CYP4F2, respectively. The remaining lanes were each loaded with 75 μg of microsomal protein from the HLM-TT (lane 6), HLM-CT (lane 7), and HLM-CC (lane 8) pools. Marker protein was prestained with fluorescent dye having an emission maximum at 680 nm (red), whereas the CYP4F2 antibody was conjugated with fluorophore IR800, which emits between 800 and 900 nm (green).