TABLE 4.
The neurosteroid 3α5αP potentiates single-channel currents from the concatemeric mutant γβαQ241L-βα receptor
Effect of 1 μM 3α5αP on currents elicited by 100 μM GABA. Mean durations and prevalence for the three open- and closed-time components are given. The data are presented as mean ± S.D. from five (GABA) or four patches (GABA + 3α5αP). The significance levels apply to comparison with the data in the absence of steroid.
| [GABA] | [3α5αP] | OT1 | Fraction OT1 | OT2 | Fraction OT2 | OT3 | Fraction OT3 | CT1 | Fraction CT1 | CT2 | Fraction CT2 | CTβ | Fraction CTβ |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| ms | ms | ms | ms | ms | ms | ||||||||
| 100 μM | 0.27 ± 0.17 | 0.16 ± 0.03 | 3.1 ± 0.6 | 0.65 ± 0.10 | 8.0 ± 2.1 | 0.19 ± 0.10 | 0.16 ± 0.02 | 0.64 ± 0.05 | 1.6 ± 0.7 | 0.12 ± 0.03 | 13 ± 2 | 0.24 ± 0.05 | |
| 100 μM | 1 μM | 0.32 ± 0.08† | 0.36 ± 0.08*** | 2.5 ± 1.1† | 0.39 ± 0.08*** | 21.4 ± 5.9** | 0.39 ± 0.08 | 0.16 ± 0.04† | 0.64 ± 0.06† | 1.3 ± 0.3* | 0.25 ± 0.06** | 12 ± 5† | 0.11 ± 0.04** |
*
P < 0.05.
**
P < 0.01.
***
P < 0.001.
†
Not significant.