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Uptake kinetics of [3H]DA in cells expressing hDAT and mutant hDAT. [3H]DA uptake was measured at indicated DA concentrations under initial rate conditions as described under Materials and Methods in hDAT(▪), T62A-hDAT (▴), and T62D-hDAT (○) cells. Kinetic analysis was performed by nonlinear regression analysis of the individual experiments for each mutant using GraphPad Prism version 4.
